Analysis Proposal
Analysis Question
Can we validate a functional relationship between CD38 and NAMPT in the context of NAD+ metabolism and neurodegeneration?
Datasets
GEO transcriptomics datasets (GSE, Alzheimer's Disease Neuroimaging Initiative transcriptomic data)Protein-protein interaction databases (STRING, BioGRID) with co-expression filtersMetabolomics repositories (MetaboLights, Human Metabolome Database)Single-cell RNA-seq datasets from neurodegeneration models
Methods
Cross-species co-expression analysis across neurodegeneration datasets to assess CD38-NAMPT transcriptional correlationEnrichment analysis of CD38-associated gene sets for NAMPT pathway membershipProtein interaction network analysis with confidence scoring using STRING APIMeta-analysis of NAD+ pathway gene co-regulation in Alzheimer's and Parkinson's disease cohorts
Expected Outputs
- Correlation coefficient for CD38-NAMPT co-expression across >=3 independent neurodegeneration datasets
- Enrichment p-value for NAMPT appearing in CD38 co-expression modules
- STRING confidence score for direct or indirect CD38-NAMPT interaction
- Evidence supporting/refuting CD38-NAMPT axis as a NAD+-regulatory mechanism in neuronal resilience