🧫 Experiment Protocol
ValidationSkin squamous cell carcinomaNTN1Primary mouse modelproposed
This experiment examined netrin-1 expression patterns in a primary mouse model of skin squamous cell carcinoma (SCC) that exhibits spontaneous epithelial-to-mesenchymal transition (EMT). The researchers used this model to investigate the role of netrin-1 in cancer progression and EMT regulation. The study found that netrin-1 was upregulated in the SCC model, suggesting its potential involvement in EMT processes and cancer progression.
PRIMARY OUTCOME
Netrin-1 expression levels in SCC
EXPECTED OUTCOMES
Upregulation of netrin-1 in SCC exhibiting spontaneous EMT
SUCCESS CRITERIA
Detection of increased netrin-1 expression in EMT-positive tumors
PROTOCOL
Primary mouse model of skin SCC with analysis of netrin-1 expression patterns