🧫 Experiment Protocol
Validationperioperative neurocognitive disordersC1qaratsproposed
This targeted intervention experiment involved neutralizing C1q, the initiator of the complement cascade, to test its role in microglial synaptic elimination following prolonged anesthesia. C1q neutralization was performed in the sevoflurane-induced neurotoxicity model to determine if blocking the complement pathway at its initiation point could prevent synaptic loss. The results showed that C1q neutralization led to decreased complement activation and interrupted synaptic elimination, providing direct evidence that the complement cascade is essential for anesthesia-induced synaptic loss.
PRIMARY OUTCOME
interruption of synaptic elimination
EXPECTED OUTCOMES
Reduced complement activation and preserved synaptic integrity
SUCCESS CRITERIA
Decreased complement system activation and interrupted synaptic elimination
PROTOCOL
C1q neutralization followed by prolonged sevoflurane anesthesia and assessment of complement activation and synaptic integrity