🧫 Experiment Protocol
ExploratoryMetabolic regulationTug (Aspscr1)Cell culture and mouse tissueproposed
Investigation of the mechanism by which the TUG C-terminal cleavage product enters the nucleus and interacts with PPARγ and PGC-1α. This study demonstrated that after insulin-stimulated cleavage and GLUT4 release, the TUG C-terminal fragment translocates to the nucleus where it binds to transcriptional regulators to control gene expression programs involved in lipid oxidation and thermogenesis.
PRIMARY OUTCOME
Nuclear localization and protein-protein interactions
EXPECTED OUTCOMES
TUG C-terminal product localizes to nucleus and binds PPARγ/PGC-1α
SUCCESS CRITERIA
Demonstration of nuclear localization and specific protein interactions
PROTOCOL
Subcellular fractionation, immunofluorescence microscopy, co-immunoprecipitation, chromatin immunoprecipitation