Vagus Nerve Alpha-Synuclein Propagation and Brainstem Involvement in PD

🧫 Experiment Protocol In-VivoParkinson diseaseC57BL/6 mice with vagotomy + enteric PFF injectionproposed

Test whether unilateral vagus nerve transection blocks retrograde propagation of enterically-administered alpha-synuclein preformed fibrils (PFFs) to the ipsilateral brainstem, reducing PD-like motor deficits and Lewy body formation.

PRIMARY OUTCOME

p-Syn (Ser129) IHC burden in DMV and motor behavioral scores

EXPECTED OUTCOMES

Vagotomized mice show >50% reduction in p-Syn (Ser129) pathology in the ipsilateral DMV and demonstrate attenuated motor impairment vs. sham-operated controls.

SUCCESS CRITERIA

p-Syn pathology scoring in DMV (IHC); motor behavioral testing (rotarod, gait analysis); threshold: p < 0.05 for both.

PROTOCOL

in-vivo

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