🧫 Experiment Protocol
ExploratoryinflammationRELinnate immune cellsproposed
Mechanistic study investigating how VLC ceramides drive persistent inflammation through the REL transcription factor. The experiment examined whether the inflammatory effects of VLC ceramide accumulation are dependent on sustained REL activity. Researchers analyzed the transcriptional program controlled by REL in the context of VLC ceramide-mediated inflammation and tested whether REL is necessary for the inflammatory phenotype.
PRIMARY OUTCOME
REL activity and inflammatory gene expression
EXPECTED OUTCOMES
VLC ceramide-driven inflammation requires sustained REL transcription factor activity
SUCCESS CRITERIA
Demonstration that REL is necessary for VLC ceramide-mediated inflammatory responses
PROTOCOL
Analysis of REL transcription factor activity, assessment of REL-dependent gene expression programs, and evaluation of REL requirement for VLC ceramide-mediated inflammation