🧫 Experiment Protocol
ExploratoryMendelian diseasesHuman tissuesproposed
Construction and analysis of tissue-specific protein interaction networks by integrating the HuRI interactome with tissue-specific gene expression data. This analysis involved inferring which protein interactions are likely to be active in different tissues based on the co-expression of the interacting proteins in specific tissues. The study examined general principles governing the formation of cellular context-specific functions and investigated how tissue-specific interaction networks might contribute to the molecular mechanisms underlying tissue-specific phenotypes observed in Mendelian diseases.
PRIMARY OUTCOME
Tissue-specific interaction networks and their functional implications
EXPECTED OUTCOMES
Identification of tissue-specific interaction patterns and elucidation of molecular mechanisms underlying tissue-specific disease phenotypes
SUCCESS CRITERIA
Successful inference of biologically relevant tissue-specific networks that correlate with known tissue-specific functions and disease phenotypes
PROTOCOL
Integration of HuRI with transcriptome data to infer tissue-specific active interactions, analysis of tissue-specific network properties, correlation with Mendelian disease phenotypes