🧫 Experiment Protocol
ExploratoryschizophreniaNr3c1cell culture system with dual-luciferase reportersproposed
An in vitro dual-luciferase reporter assay was conducted to experimentally validate the predicted interaction between miR-137-3p and Nr3c1 mRNA. This functional assay was designed to confirm the in silico prediction that miR-137-3p regulates Nr3c1 gene expression. The dual-luciferase system allows for direct measurement of microRNA-mediated regulation of target mRNA by comparing luciferase activity in the presence and absence of the microRNA. This experiment served as a crucial validation step to confirm the computational predictions regarding miR-137-3p regulation of Nr3c1 expression changes observed in the haloperidol-exposed offspring hippocampus.
PRIMARY OUTCOME
miRNA-target interaction validation
EXPECTED OUTCOMES
Confirmation of miR-137-3p regulation of Nr3c1 expression
SUCCESS CRITERIA
Significant change in luciferase activity indicating miRNA-target interaction
PROTOCOL
Dual-luciferase reporter assay with miR-137-3p and Nr3c1 target sequences