Enteric Alpha-Synuclein Propagation and ENS Dysfunction in PD Mouse Model

🧫 Experiment Protocol In-VivoParkinson diseaseMouse (C57BL/6 or Thy1-αSyn transgenic), gut injection modelproposed

Determine whether alpha-synuclein fibrils injected into the gut lumen propagate via the vagus nerve to the brainstem and whether ENS dysfunction constitutes a self-reinforcing feedback loop in a preformed PD mouse model.

PRIMARY OUTCOME

p-Syn (Ser129) pathology burden in vagus nerve and brainstem DMV

EXPECTED OUTCOMES

Alpha-synuclein pathology detected in vagus nerve and dorsal motor nucleus by 6 months post-injection, with progressive ENS dysfunction evidenced by delayed gut transit.

SUCCESS CRITERIA

p-Syn (Ser129) IHC in vagus and DMV; threshold: pathology score > 2 in treatment vs. control groups.

PROTOCOL

in-vivo

Source: auto-generated