Single nucleus RNA-seq and ATAC-seq of postmortem PD brain tissue (n=19) and controls (n=14) identified 13 microglial subpopulations; CD83+ HIF1A- microglia were specifically depleted in substantia nigra in PD with unique chromatin signature, and this population normally shows brainstem regional spe

🧫 Experiment Protocol Gene ExpressionParkinson's diseaseCD83postmortem_human_tissueextracted

10x Genomics Chromium platform for single nucleus multiome RNA-seq and ATAC-seq; four brain regions analyzed to compare neurodegeneration severity

PRIMARY OUTCOME

Single nucleus RNA-seq and ATAC-seq of postmortem PD brain tissue (n=19) and controls (n=14) identified 13 microglial subpopulations; CD83+ HIF1A- microglia were specifically depleted in substantia nigra in PD with unique chromatin signature, and this population normally shows brainstem regional specificity.

LINKED HYPOTHESES

h-cc60dcd54d · H2S/butyrate imbalance drives enteric alpha-synuclein pathology via TLR4 signaling

Source: pmid_37292857