🧫 Experiment Protocol
In-VitroNeurodegeneration (AD/PD)iPSC-derived microglia-astrocyte-neuron triple co-culturecompleted
Elucidate the molecular mechanisms by which TREM2 expressed on microglia mediates beneficial cross-talk with astrocytes, and how this axis is disrupted in TREM2 deficiency models of neurodegeneration.
PRIMARY OUTCOME
Astrocyte neuroprotective marker panel and neuron survival under oxidative stress
EXPECTED OUTCOMES
TREM2 knockdown in microglia reduces astrocyte activation toward neuroprotective phenotype (reduced GFAP, increased S100A10), and co-culture supernatant is less protective against oxidative stress injury.
SUCCESS CRITERIA
Astrocyte phenotype markers (RT-qPCR, ELISA: GFAP, S100A10, IL-10); neuron survival after oxidative stress (MTT/WST-8); threshold: p < 0.05.
Source: auto-generated