🧫 Experiment Protocol
In-VivoAlzheimer diseaseMouse (5xFAD or kcng1-tau model) with chronic implanted electrodes/optrodesproposed
Determine whether closed-loop optogenetic PV interneuron stimulation can restore theta-gamma coupling and improve spatial memory in a mouse model of AD or epilepsy with documented coupling deficits.
PRIMARY OUTCOME
LFP theta-gamma coupling and spatial memory performance (MWM)
EXPECTED OUTCOMES
Closed-loop stimulation restores theta-gamma coupling (phase-amplitude coupling index restored to >80% of baseline) and improves spatial memory performance (Morris water maze) vs. open-loop or no-stimulation controls.
SUCCESS CRITERIA
LFP theta-gamma PAC (phase-amplitude coupling) and Morris water maze latency; threshold: p < 0.05 for both.
Source: auto-generated