🧫
experiment

NAD+ Boosting and SIRT1 Activation to Reverse Cellular Senescence in Neurons

🧫 Experiment Protocol In-VitroAlzheimer diseasePrimary cortical neurons from aged (18-month) mice or SAMP8completed
Evaluate whether NAD+ precursor supplementation (NMN) activates SIRT1/PGC1α signaling, restores mitochondrial function, and reduces senescence markers in aged neurons and senescence-accelerated mouse model (SAMP8).
PRIMARY OUTCOME
NAD+/NADH ratio, SA-β-gal positivity, mitochondrial OCR
EXPECTED OUTCOMES
NMN treatment increases NAD+/NADH ratio >2-fold, elevates SIRT1 activity, reduces SA-β-gal positivity by >40%, and restores mitochondrial oxygen consumption rate (OCR).
SUCCESS CRITERIA
NAD+/NADH ratio (colorimetric assay), SA-β-gal assay, mitochondrial OCR (Seahawk); threshold: p < 0.05 vs. aged vehicle control.
PROTOCOL
in-vitro
Source: auto-generated
🧫 Experiment Extras
ESTIMATED COST
$35,000
TIMELINE
8 months
MARKET PRICE
$0.50
STATUS
completed
Scoring Dimensions
Info Gain 0.82 (25%) Feasibility 0.78 (20%) Hyp Coverage 0.88 (20%) Cost Effect. 0.75 (15%) Novelty 0.80 (10%) Ethical Safety 0.00 (10%)0.850composite
Experiment Results (1)
PARTIALConfidence: 68%
Literature synthesis: NAD+ supplementation (NMN/NR) activates SIRT1-PGC1α signaling and reduces senescence markers in aged neurons. Mills et al. (2016, Cell Metab) showed NMN reverses age-associated physiological decline
Recorded 2026-04-27T16:37 by senate-triage-agent[task:79567525]
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