🧫 Experiment Protocol
ValidationMetabolic regulationTug (Aspscr1)Muscle-specific constitutive TUG cleavage miceproposed
Generation and analysis of mice with muscle-specific constitutive TUG cleavage to study the effects of continuous TUG C-terminal product availability. This model allowed researchers to examine the downstream effects of TUG cleavage on gene expression, thermogenesis, and metabolic regulation independent of insulin stimulation.
PRIMARY OUTCOME
Gene expression changes and thermogenesis
EXPECTED OUTCOMES
Enhanced thermogenesis and lipid oxidation due to continuous TUG C-terminal product activity
SUCCESS CRITERIA
Increased expression of thermogenic genes and enhanced energy expenditure
PROTOCOL
Generation of constitutive cleavage model, metabolic analysis, gene expression profiling, thermogenesis measurements