🧫 Experiment Protocol
Validationretinitis pigmentosaPde6brd10 miceproposed
Intravitreal injections of IGF-I were administered to rd10 mice to evaluate neuroprotective effects on photoreceptor degeneration in vivo. The experiment assessed photoreceptor cell death using TUNEL staining in retinal sections and quantified free nucleosomes in retinal extracts by ELISA. Microglial cell number and distribution were analyzed using immunolabeling with Cd11b and Iba1 antibodies. The study demonstrated that in vivo IGF-I treatment significantly reduced the number of TUNEL-positive nuclei in rd10 retinas, confirming the neuroprotective effects observed in the ex vivo experiments.
PRIMARY OUTCOME
photoreceptor cell death reduction
EXPECTED OUTCOMES
IGF-I would provide neuroprotection against photoreceptor degeneration in vivo
SUCCESS CRITERIA
Reduction in TUNEL-positive nuclei and free nucleosome levels
PROTOCOL
Intravitreal injection of IGF-I, TUNEL staining of retinal sections, ELISA quantification of free nucleosomes in retinal extracts, microglial immunolabeling with Cd11b and Iba1