🧫 Experiment Protocol
ExploratoryMetabolic regulationSarcolipin, UCP1, Tug (Aspscr1)Mouse muscle and adipose tissueproposed
Analysis of how TUG cleavage regulates the expression of thermogenic genes including sarcolipin in muscle and uncoupling protein 1 (UCP1) in adipose tissue. The study examined the transcriptional changes downstream of TUG cleavage that promote lipid oxidation and thermogenesis, demonstrating that this pathway coordinates glucose uptake with energy expenditure.
PRIMARY OUTCOME
Expression levels of thermogenic genes
EXPECTED OUTCOMES
Upregulation of sarcolipin and UCP1 following TUG cleavage
SUCCESS CRITERIA
Significant increases in thermogenic gene expression
PROTOCOL
RNA extraction, quantitative PCR, RNA sequencing, gene expression analysis