🧫 Experiment Protocol
ExploratoryMendelian diseasesYeast two-hybrid systemproposed
A comprehensive systematic mapping of human binary protein-protein interactions using a proteome-wide approach. The study employed high-throughput yeast two-hybrid screening to identify direct physical interactions between human proteins. This represents the most comprehensive 'all-by-all' screen of human protein interactions to date, generating approximately 53,000 protein-protein interactions, which is approximately four times larger than existing high-quality curated interaction datasets from small-scale studies. The interactome was systematically validated and integrated with genomic, transcriptomic, and proteomic data to enable functional studies across different cellular contexts.
PRIMARY OUTCOME
Comprehensive human protein-protein interaction map
EXPECTED OUTCOMES
Generation of a comprehensive reference interactome that would enable linking genomic variation to phenotypic outcomes and reveal tissue-specific interaction networks
SUCCESS CRITERIA
Detection of high-quality binary protein interactions with validation across multiple experimental approaches
PROTOCOL
High-throughput yeast two-hybrid screening of human protein pairs, systematic validation of interactions, integration with multi-omics data including genome, transcriptome and proteome datasets