🧫 Experiment Protocol
Exploratoryamyotrophic lateral sclerosisTARDBPcultured cellsproposed
Investigation of how TDP-43 protein invades mitochondria and triggers mitochondrial DNA (mtDNA) release through the mitochondrial permeability transition pore (mPTP). The study examined the mechanism by which cytoplasmic TDP-43 accumulation leads to mitochondrial dysfunction and subsequent mtDNA release into the cytoplasm, which then activates innate immune signaling pathways.
PRIMARY OUTCOME
mitochondrial DNA release
EXPECTED OUTCOMES
TDP-43 invasion of mitochondria triggers mPTP opening and subsequent mtDNA release
SUCCESS CRITERIA
Detection of mtDNA in cytoplasm following TDP-43 mitochondrial invasion
PROTOCOL
Assessment of TDP-43 localization to mitochondria, measurement of mitochondrial permeability transition pore opening, and detection of cytoplasmic mtDNA release