🧫 Experiment Protocol
ValidationDiabetes/metabolic dysfunctionTug (Aspscr1)Muscle-specific Tug knockout miceproposed
Generation and analysis of muscle-specific Tug (Aspscr1) knockout mice to investigate the role of TUG cleavage in glucose uptake and energy expenditure. The study examined how loss of TUG affects GLUT4 translocation, glucose uptake in muscle, and whole-body metabolic parameters including thermogenesis and lipid oxidation.
PRIMARY OUTCOME
Glucose uptake and energy expenditure
EXPECTED OUTCOMES
Reduced glucose uptake and altered energy expenditure due to loss of TUG cleavage pathway
SUCCESS CRITERIA
Measurable changes in glucose uptake and metabolic parameters
PROTOCOL
Generation of muscle-specific knockout mice, metabolic phenotyping, glucose uptake assays, gene expression analysis