SASP Factor Secretion Patterns and Their Impact on Neuronal Health

🧫 Experiment Protocol In-VitroAlzheimer diseasePrimary astrocytes from aged donor tissue + primary cortical neuronsproposed

Characterize the secretome (SASP) profile of senescent astrocytes and fibroblasts from aged brain tissue, and test whether pharmacological SASP modulation (e.g., rapamycin, navitoclax) preserves neuronal health in co-culture.

PRIMARY OUTCOME

Neuronal viability and SASP cytokine panel in co-culture

EXPECTED OUTCOMES

SASP-modulated co-cultures show >40% improvement in neuronal survival under stress conditions vs. untreated senescent co-cultures.

SUCCESS CRITERIA

Multiplex cytokine array (IL-6, IL-8, TNF-α, etc.), neuronal viability (Live/Dead assay); threshold: p < 0.05 for survival outcome.

PROTOCOL

in-vitro

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