🧫 Experiment Protocol
ValidationAlzheimer's diseasemiceproposed
Parallel to the human studies, bacterial extracellular vesicles were extracted from mouse fecal and blood samples to measure LPS content. This animal model component provided a controlled experimental system to validate findings from human samples and potentially investigate mechanisms of bEV transport and function in a laboratory setting where variables could be better controlled.
PRIMARY OUTCOME
LPS levels in bacterial extracellular vesicles
EXPECTED OUTCOMES
Detectable LPS-containing bEVs in mouse samples
SUCCESS CRITERIA
Successful isolation and quantification of LPS in mouse-derived bEVs
PROTOCOL
Extraction of bEVs from mouse feces and blood samples, measurement of LPS levels