TLR4-NF-κB-NLRP3 Axis Inhibition in iPSC-derived Microglia for AD Prevention

🧫 Experiment Protocol In-VitroAlzheimer diseaseiPSC-derived microglia-neuron co-culture from AD patientsproposed

Evaluate whether selective TLR4-NF-κB-NLRP3 axis inhibition reduces neuroinflammatory burden and prevents neuronal loss in Alzheimer disease iPSC-derived microglia-neuron co-culture models.

PRIMARY OUTCOME

NLRP3 inflammasome activation markers (IL-1β, IL-18) and neuronal viability (TUNEL/cleaved caspase-3)

EXPECTED OUTCOMES

Reduction in NLRP3 inflammasome activation (IL-1β, IL-18 release) by >50% and decreased neuronal apoptosis in co-culture.

SUCCESS CRITERIA

NLRP3 activation assay (ELISA) + TUNEL staining neuronal viability; threshold: p < 0.05 vs. LPS-stimulated control.

PROTOCOL

in-vitro

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