🧫 Experiment Protocol
ExploratoryAlzheimer's diseaseTARDBPhuman postmortem brain tissueproposed
Neuropathological examination of Alzheimer's disease cases to determine the prevalence and distribution pattern of TDP-43 inclusions. The study found that TDP-43 inclusions occur in up to 57% of AD cases, most commonly in a limbic distribution pattern, with or without associated hippocampal sclerosis. The inclusions consist of phosphorylated and truncated forms of TDP-43 protein forming cytoplasmic inclusion bodies. In some cases, TDP-43 deposits were observed co-localizing with neurofibrillary tangles in neurons, suggesting potential interaction between tau and TDP-43 pathologies.
PRIMARY OUTCOME
prevalence and distribution of TDP-43 pathology
EXPECTED OUTCOMES
characterization of TDP-43 pathology frequency and anatomical distribution in AD
SUCCESS CRITERIA
detection and quantification of TDP-43 inclusions in brain regions
PROTOCOL
neuropathological examination of AD brain tissue using immunohistochemistry for phosphorylated TDP-43