🧫 Experiment Protocol
ClinicalCOVID-19human peripheral blood from COVID-19 patientsproposed
Proteomic analysis of blood samples from COVID-19 patients to measure levels of purinergic signaling molecules including ATP, ADP, AMP, inosine, and hypoxanthine using mass spectrometry. The study examined correlations between these nucleotides/nucleosides and coagulation proteins (fibrinogen and other coagulation factors) as well as clinical features. Blood metabolite levels were compared between COVID-19 patients at different disease stages and healthy controls or non-COVID-19 patients. The analysis revealed stage-dependent changes in metabolite levels and significant correlations with coagulation markers.
PRIMARY OUTCOME
levels of purinergic metabolites and correlation with coagulation proteins
EXPECTED OUTCOMES
Altered purinergic metabolite levels in COVID-19 patients with correlations to coagulation markers
SUCCESS CRITERIA
Significant changes in metabolite levels and correlations with coagulation proteins
PROTOCOL
Raw proteomics data acquired using mass spectrometry from public datasets (GEO), analyzed for ATP, ADP, AMP, inosine, hypoxanthine levels and correlations with fibrinogen and coagulation proteins