Single nuclei multiomic profiling (transcriptomes, epigenomes, spatial coordinates) of 84 human donors spanning AD pathology spectrum mapped to a common cell type reference. Analysis revealed early reduction of Somatostatin-expressing neuronal subtypes and late decreases in supragranular intratelenc

SUMMARY

Single nuclei multiomic profiling (transcriptomes, epigenomes, spatial coordinates) of 84 human donors spanning AD pathology spectrum mapped to a common cell type reference. Analysis revealed early reduction of Somatostatin-expressing neuronal subtypes and late decreases in supragranular intratelencephalic-projecting excitatory and Parvalbumin-expressing neurons, with increases in disease-associated microglial and astrocytic states.

METHODOLOGY NOTES

Image-based quantitative neuropathology used to create continuous disease pseudoprogression score; single nuclei multiomic profiling with transcriptome and epigenome measurement; spatial transcriptomics; BRAIN Initiative Cell Census Network methodology; publicly available resource at SEA-AD.org