🧪
hypothesis

Fyn-anchored dendritic tau/NMDAR signaling persists after transient Aβ exposure

Hypothesis

Fyn-anchored dendritic tau/NMDAR signaling persists after transient Aβ exposure

Aβ drives tau into dendritic spines, where tau binds Fyn and stabilizes a PSD95-NMDAR-associated excitotoxic scaffold.
🧬 MAPT,FYN,DLG4,GRIN2B🩺 neurodegeneration🎯 Composite 67%💱 $0.58▼14.1%proposed
EvidencePending (0%)📖 0 cit🗣 1 debates 3 support 2 oppose
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Mechanistic 0.80 (15%) Evidence 0.71 (15%) Novelty 0.58 (12%) Feasibility 0.79 (12%) Impact 0.66 (12%) Druggability 0.63 (10%) Safety 0.50 (8%) Competition 0.57 (6%) Data Avail. 0.76 (5%) Reproducible 0.70 (5%) KG Connect 0.50 (8%) 0.670 composite
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Composite67%

🧪 Overview

Aβ drives tau into dendritic spines, where tau binds Fyn and stabilizes a PSD95-NMDAR-associated excitotoxic scaffold. Once assembled, this complex may persist after Aβ clearance and maintain calcium dysregulation, hyperexcitability, and synaptic degeneration.

🧬 Mechanism

🧬 Curated Mechanism Pathway

Curated pathway from expert analysis

flowchart TD
    A["MAPT<br/>Primary Target"]
    B["Biological Process 1<br/>Mechanistic Step A"]
    C["Biological Process 2<br/>Mechanistic Step B"]
    D["Output Phenotype<br/>Disease Effect"]
    A --> B
    B --> C
    C --> D
    style A fill:#1a237e,stroke:#4fc3f7,color:#4fc3f7
    style D fill:#b71c1c,stroke:#ef9a9a,color:#ef9a9a

⚖️ Evidence

⚖️ Evidence Matrix3 supports2 contradicts
Supports
Dendritic tau mediates Aβ toxicity via Fyn-dependent NMDA receptor signaling, strongly supporting this signaling axis.
PMID:20655099
Supports
Aβ oligomers induce tau missorting, local calcium rise, and spine loss, consistent with a feed-forward excitotoxic framework.
PMID:20826658
Supports
Soluble Aβ oligomers drive tau mislocalization to spines and receptor-signaling deficits.
PMID:24713000
Contradicts
Existing evidence mainly shows that tau is required for Aβ toxicity, not that the tau-Fyn scaffold persists once Aβ is fully absent.
PMID:20655099
Contradicts
Persistent calcium dysregulation could reflect irreversible spine injury or residual Aβ rather than a self-maintained tau-Fyn complex.
PMID:24713000
📖 Linked Papers

No linked papers recorded for this hypothesis yet.

🏥 Translation

🧬 3D Protein Structure — MAPT

🧬 PDB 5O3L Click to expand

Experimental structure from RCSB PDB | Powered by Mol*

🧠 GTEx v10 Brain ExpressionJSON

Median TPM across 13 brain regions for MAPT,FYN,DLG4,GRIN2B from GTEx v10.

Cerebellum209 Cerebellar Hemisphere199 Cortex152 Frontal Cortex BA9146 Anterior cingulate cortex BA24101 Hypothalamus86.4 Amygdala73.5 Nucleus accumbens basal ganglia72.2 Hippocampus72.1 Caudate basal ganglia64.7 Putamen basal ganglia58.1 Substantia nigra56.8 Spinal cord cervical c-149.2median TPM (GTEx v10)

💉 Clinical Trials

No clinical trials data linked to this hypothesis yet.

No curated ClinVar variants loaded for this hypothesis.

Run scripts/backfill_clinvar_variants.py to fetch P/LP/VUS variants.

🔍 Search ClinVar for MAPT,FYN,DLG4,GRIN2B →

No DepMap CRISPR Chronos data found for MAPT,FYN,DLG4,GRIN2B.

Run python3 scripts/backfill_hypothesis_depmap.py to populate.

💰 Estimated Development
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🔮 Predictions

🔎 Predictions vs Observations2 predictions · 0 with recorded observations
PredictionPredictedObservedStatusConf
IF wild‑type mice receive a single intracerebroventricular injection of Aβ1‑42 oligomers (300 pmol) followed by a 7‑day Aβ‑free period, THEN the amount of Fyn‑tau‑PSD95‑NMDAR complex recovered from sy2‑fold increase in co‑immunoprecipitated Fyn/tau/PSD95/GRIN2B and a ≥30 % rise in basal calcium fluorescence (GCaMP6f) in CA1 pyramidal neurons.— no observation —pending0.65
IF human iPSC‑derived cortical neurons are exposed to 200 nM Aβ1‑42 oligomers for 48 h, THEN after a 96‑h Aβ‑free interval the tau Y18 phosphorylation (Fyn site) co‑localized with PSD95 will stay >1.5≥1.5‑fold increase in PLA puncta for pTau(Y18)–PSD95 and ≥40 % decrease in mEPSC frequency.— no observation —pending0.60
🔮 Falsifiable Predictions (2)
pendingconf 65%
IF wild‑type mice receive a single intracerebroventricular injection of Aβ1‑42 oligomers (300 pmol) followed by a 7‑day Aβ‑free period, THEN the amount of Fyn‑tau‑PSD95‑NMDAR complex recovered from synaptoneurosomes will remain at least 2‑fold higher than in vehicle‑treated controls, AND hippocampal
Predicted outcome: 2‑fold increase in co‑immunoprecipitated Fyn/tau/PSD95/GRIN2B and a ≥30 % rise in basal calcium fluorescence (GCaMP6f) in CA1 pyramidal neurons.
Falsification: Complex abundance returns to ≤1.2‑fold of control AND calcium fluorescence is unchanged (<10 % difference), indicating no persistence.
pendingconf 60%
IF human iPSC‑derived cortical neurons are exposed to 200 nM Aβ1‑42 oligomers for 48 h, THEN after a 96‑h Aβ‑free interval the tau Y18 phosphorylation (Fyn site) co‑localized with PSD95 will stay >1.5‑fold above baseline, AND miniature excitatory postsynaptic current (mEPSC) frequency will remain re
Predicted outcome: ≥1.5‑fold increase in PLA puncta for pTau(Y18)–PSD95 and ≥40 % decrease in mEPSC frequency.
Falsification: pTau(Y18)–PSD95 PLA signal returns to ≤1.2‑fold baseline OR mEPSC frequency is unchanged (<15 % reduction), indicating no persistent signaling.
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