Restoration of Neuronal Ketone Body Utilization via MCT1 Upregulation
Curated pathway from expert analysis
flowchart TD
A["SLC16A1 MCT1<br/>Upregulation"]
B["Ketone Body<br/>Neuronal Import"]
C["Neuronal Energy<br/>Metabolism Restoration"]
D["Mitochondrial<br/>Function Support"]
E["Neuroprotective<br/>Energy State"]
A --> B
B --> C
C --> D
D --> E
style A fill:#1b5e20,stroke:#a5d6a7,color:#a5d6a7
style E fill:#1b5e20,stroke:#a5d6a7,color:#a5d6a7No linked papers recorded for this hypothesis yet.
No curated PDB or AlphaFold mapping for SLC16A1 yet. Search RCSB →
Median TPM across 13 brain regions for SLC16A1 (MCT1) from GTEx v10.
No clinical trials data linked to this hypothesis yet.
No curated ClinVar variants loaded for this hypothesis.
Run scripts/backfill_clinvar_variants.py to fetch P/LP/VUS variants.
No DepMap CRISPR Chronos data found for SLC16A1 (MCT1).
Run python3 scripts/backfill_hypothesis_depmap.py to populate.
| Prediction | Predicted | Observed | Status | Conf |
|---|---|---|---|---|
| IF 3-month-old 3xTg-AD mice are treated with chronic MCT1 agonist (α-cyano-4-hydroxycinnamate at 10 mg/kg/day via osmotic pump) for 8 weeks, THEN brain tissue β-hydroxybutyrate concentration will incr | Brain BHB concentration increases from ~0.8 μmol/g to ≥1.12 μmol/g; latency to platform decreases from baseline ~45 sec to ≤33 sec on day 5 of Morris water maze | — no observation — | pending | 0.45 |
| IF human iPSC-derived neurons are transduced with SLC16A1 (MCT1) overexpression vector (AAV9-hSLC16A1), THEN 13C-beta-hydroxybutyrate uptake will increase by at least 50% within 72 hours compared to A | Neuronal 13C-BHB uptake rate increases from baseline ~2.5 nmol/mg protein/min to ≥3.75 nmol/mg protein/min (50% increase); intracellular ketone body metabolite | — no observation — | pending | 0.65 |