Phosphatidylserine Decarboxylase (PISD) Restoration to Correct Mitochondrial Membrane PS Asymmetry in AD Neurons
Curated pathway from expert analysis
flowchart TD
A["PISD Restoration<br/>Phosphatidylserine Decarboxylase"]
B["Mitochondrial Membrane<br/>PS Asymmetry Correction"]
C["Apoptosis Sensitivity<br/>Restored to Normal"]
D["Cell Death Threshold<br/>Properly Calibrated"]
E["Neuronal Survival<br/>Enhanced"]
F["PISD as<br/>Mitochondrial Integrity Target"]
A --> B
B --> C
C --> D
D --> E
E --> F
style A fill:#1a237e,stroke:#4fc3f7,color:#4fc3f7
style F fill:#1b5e20,stroke:#a5d6a7,color:#a5d6a7No linked papers recorded for this hypothesis yet.
No curated PDB or AlphaFold mapping for PISD yet. Search RCSB →
Median TPM across 13 brain regions for PISD from GTEx v10.
No clinical trials data linked to this hypothesis yet.
No curated ClinVar variants loaded for this hypothesis.
Run scripts/backfill_clinvar_variants.py to fetch P/LP/VUS variants.
No DepMap CRISPR Chronos data found for PISD.
Run python3 scripts/backfill_hypothesis_depmap.py to populate.
| Prediction | Predicted | Observed | Status | Conf |
|---|---|---|---|---|
| IF PISD restoration normalizes mitochondrial PS asymmetry in AD neurons THEN neuronal basal oxygen consumption rate (OCR) will increase by at least 20 % within 4 weeks of PISD overexpression. | A ≥20 % rise in basal mitochondrial OCR compared with vehicle‑treated AD neurons. | — no observation — | pending | 0.65 |
| IF PISD is overexpressed via AAV-mediated transduction in primary cortical neurons derived from 5xFAD AD mice THEN mitochondrial membrane phosphatidylserine asymmetry, as quantified by flow cytometric | At least a 30 % decrease in external phosphatidylserine on isolated brain mitochondria, measured by annexin V positivity. | — no observation — | pending | 0.65 |