Cerebral microvascular endothelial cells shed submicron microparticles (EMPs) upon activation or apoptosis. EMPs carry surface markers reflecting parent cell state—CD31, CD105, and LRP1. Analyzing circulating EMP populations via flow cytometry provides a real-time snapshot of cerebral endothelial status. However, flow cytometry standardization across laboratories is lacking, and pre-analytical variables dramatically affect EMP counts.
Curated pathway from expert analysis
flowchart TD
A["PECAM1<br/>Platelet Endothelial Cell Adhesion"]
B["Endothelial<br/>Microparticles"]
C["Circulating EMPs<br/>CD31+/CD144+"]
D["Vascular<br/>Endothelial Activation"]
E["BBB<br/>Dysfunction"]
F["Prothrombotic<br/>State"]
G["Neurovascular<br/>Compromise"]
H["Cognitive<br/>Impairment"]
A --> B
B --> C
C --> D
D --> E
E --> F
F --> G
G --> H
style A fill:#1a237e,stroke:#4fc3f7,color:#4fc3f7
style F fill:#b71c1c,stroke:#ef9a9a,color:#ef9a9a
style H fill:#b71c1c,stroke:#ef9a9a,color:#ef9a9aNo linked papers recorded for this hypothesis yet.
No curated PDB or AlphaFold mapping for PECAM1 yet. Search RCSB →
Median TPM across 13 brain regions for PECAM1 from GTEx v10.
No clinical trials data linked to this hypothesis yet.
No curated ClinVar variants loaded for this hypothesis.
Run scripts/backfill_clinvar_variants.py to fetch P/LP/VUS variants.
No DepMap CRISPR Chronos data found for PECAM1.
Run python3 scripts/backfill_hypothesis_depmap.py to populate.
No resource usage or linked notebooks recorded for this hypothesis yet.
| Prediction | Predicted | Observed | Status | Conf |
|---|---|---|---|---|
| IF we compare circulating EMP phenotypes (activated LRP1+/CD31+ EMP count per μL plasma) between cognitively normal individuals at genetic risk for neurodegeneration (APOE4 homozygous, n≥50) and age-m | Activated LRP1+/CD31+ EMP count significantly higher in neurodegeneration-risk group vs. non-neurodegenerative inflammation group. | — no observation — | pending | 0.65 |
| IF we stratify cognitively normal adults aged 60-80 years into quartiles based on baseline circulating activated LRP1+/CD31+ EMP count (standardized flow cytometry), THEN the highest quartile (Q4) wil | MCI/dementia incidence in Q4 ≥15% vs. Q1 ≤7.5% within 3 years. | — no observation — | pending | 0.58 |