PLIN2 (Perilipin-2) Degradation via Autophagy Activation to Clear Disease-Associated Lipid Droplets
Curated pathway from expert analysis
flowchart TD
A["Testosterone/ANDROGEN RECEPTOR Axis<br/>Neuronal Androgen Binding"]
B["AR Nuclear Translocation<br/>Coactivator Recruitment and Hormonal Ligand"]
C["TM4SF5 and CD82 Expression<br/>Senescent Cell Surface Marker Induction"]
D["Senolytic Target Engagement<br/>p53-Dependent Apoptosis in SASP Cells"]
E["Inflammatory Niche Remodeling<br/>SASP Factor Clearance"]
F["Neurodegenerative Niche Improvement<br/>Reduced Inflammatory Tone"]
A --> B
B --> C
C --> D
D --> E
E --> F
style A fill:#1a237e,stroke:#4fc3f7,color:#4fc3f7
style D fill:#b71c1c,stroke:#ef9a9a,color:#ef9a9a
style F fill:#1b5e20,stroke:#81c784,color:#81c784No linked papers recorded for this hypothesis yet.
No curated PDB or AlphaFold mapping for PLIN2 yet. Search RCSB →
Median TPM across 13 brain regions for PLIN2/NEDD4L (Lipophagy) from GTEx v10.
No clinical trials data linked to this hypothesis yet.
No curated ClinVar variants loaded for this hypothesis.
Run scripts/backfill_clinvar_variants.py to fetch P/LP/VUS variants.
No DepMap CRISPR Chronos data found for PLIN2.
Run python3 scripts/backfill_hypothesis_depmap.py to populate.
| Prediction | Predicted | Observed | Status | Conf |
|---|---|---|---|---|
| IF autophagy is pharmacologically activated in lipid-loaded hepatocytes using 100mM trehalose for 48 hours THEN PLIN2 protein levels will decrease by at least 40% and lipid droplet area per cell will | PLIN2 protein level decreases by ≥40% (measured by Western blot normalized to β-actin); lipid droplet area decreases by ≥30% (measured by Oil Red O or BODIPY st | — no observation — | pending | 0.65 |
| IF NEDD4L is knocked down via siRNA transfection in lipid-loaded hepatocytes THEN PLIN2 protein levels will increase by at least 50% and lipid droplet area per cell will increase by at least 35% compa | PLIN2 protein level increases by ≥50% (measured by Western blot normalized to β-actin); lipid droplet area increases by ≥35% (measured by Oil Red O or BODIPY st | — no observation — | pending | 0.55 |