OBJECTIVE AND DESIGN: To define the role of Ten-Eleven Translocation (TET) proteins in Crohn's disease (CD)-associated inflammation through integrative human and mechanistic studies. MATERIAL: Publicly available CD transcriptomic and DNA methylation datasets, and primary mononuclear cells and ileal biopsies were analyzed for TET gene expression and signatures. TET1 and TET2 CRISPR/Cas9 knockout macrophage cell lines were generated. TREATMENT: Macrophages were stimulated with LPS in the presence ...