The tools for subjecting cells to high-magnitude shear stress within conventional cell culture dishes and well plates on an orbital shaking platform have remained unchanged for the past 30 years. Here, we develop a pipeline for creating custom cell culture dishes and well plates of arbitrary size and complexity using 3D printing technology. We describe two methods: direct 3D printing and reversible bonding of ACLAR film to 3D-printed structures. We show that the custom chambers support alignment...