Fig. 1Figure 1
SPP1 upregulation at onset of microglia–synapse phagocytosis. a , Representative 3D reconstructed images showing Homer1 engulfment within CD68 + lysosomes of P2Y12 + microglia in 6-month App WT (WT) versus App NL-F CA1 hippocampal SLM. Scale bar represents 5 µm. b , Quantification of Homer1 engulfment in 6-month WT and App NL-F P2Y12 + microglia. One datapoint represents average of 1 mouse from n = 4 animals per genotype examined over two independent experiments. P values from two-tailed unpaired Student’s t -test with Welch’s correction. c , Representative confocal images of C1q protein expression in 6-month WT versus App NL-F mice. Insert represents C1qa mRNA within Tmem119 + microglia. Scale bar represents 20 µm. Data are representative of two mice per genotype examined over at least five independent experiments. d , Quantification of C1q puncta in 6-month WT and App NL-F CA1 hippocampus. One datapoint represents one ROI per mouse from n = 9 WT mice and n