Fig. 3Figure 3
Spp1 modulates complement activation and microglial synaptic engulfment upon acute oAβ challenge. a , Scheme illustrating ICV injection of S26C oAβ versus PBS in WT versus Spp1 KO/KO mice, 18 h before tissue collection and analysis. b , Quantification of SPP1 immunoreactivity within SLM of 3-month WT mice injected with oAβ versus PBS control, at either 18 h or 72 h post-ICV injection. One datapoint represents one ROI per mouse hippocampus, with total of n = 3 mice per genotype and time point, examined over one independent experiment. P values from two-way ANOVA, Bonferroni’s multiple comparison test. c , Representative images of C1q expression in SLM of 3-month PBS versus oAβ-injected WT mice. Scale bar represents 20 µm. d , Representative 3D reconstructed images showing Homer1 engulfment within CD68 + lysosomes of P2Y12 + microglia from WT mice injected with oAβ. Scale bar represents 5 µm. e , Representative images of C1q expression in Spp1 KO/KO mice injected with PBS