Fig. 4Figure 4
SPP1 drives microglial engulfment of synapses in AD context. a – d , Representative image of 3D reconstructed P2Y12 + microglia expressing phagocytic markers Ctsb ( a , b ) and Grn ( c , d ) assessed by smFISH-IHC in 6-month WT, Spp1 KO/KO , App NL-F and App NL-F · Spp1 KO/KO SLM. Scale bar represents 7 µm. Quantification of Ctsb ( b ) and Grn ( d ) mRNA levels expression within P2Y12 + microglia. One datapoint represents average of 1 mouse, calculated from 6 individual P2Y12 + microglia per mouse ( b ) and 3–6 microglia ( d ) from n = 3 mice per genotype examined over 2 independent experiments. P Values from two-way ANOVA, Bonferroni’s multiple comparison test for ( b ). e , Representative 3D reconstructed images showing Homer1 engulfment within CD68 + lysosomes of P2Y12 + microglia in 6-month App NL-F versus App NL-F · Spp1 KO/KO SLM. Scale bar represents 7 µm. f , Quantification of Homer1 engulfment ratio in P2Y12 + microglia of WT versus Spp1 KO/KO ver