Extended Data Fig. 1Figure 6
Pre-plaque vascular Aβ deposition in App NL-F mice. (a , b) Quantification of SPP1 immunoreactivity in CA1 hippocampus (a) versus cerebellum as control region (b) , as measured by confocal imaging. 1 datapoint represents 1 ROI per mouse hippocampus, with total of 8 (CA1, a ) and 7 (CRB, b ) mice per genotype examined over 2 independent experiments. P Values from linear mixed-effect model with multivariate t-distribution post-hoc test. Data are shown as Mean ± SEM. (c) Validation of SPP1 antibody (left) and anti- Spp1 probe for smFISH (right) in 3 mo Spp1 KO/KO hippocampal tissue. Scale bar represents 10 µm. Data representative of n = 4 Spp1 KO/KO mice examined over 1 independent experiment. (d) Representative confocal image of Spp1 mRNA expression along GLUT1 + vasculature in SLM of 6 mo App NL-F mice as characterized by smFISH-IHC. Scale bar represents 20 µm. Insert: 3D reconstruction. Scale bar represents 5 µm. Data are representative of 3 App NL-F mice exa