Extended Data Fig. 2Figure 7
Spp1 is expressed in perivascular cells. (a) Representative confocal image showing Spp1 and Tmem119 mRNA expression within P2Y12 + microglia of 6 mo App NL-F SLM as characterized by smFISH-IHC. Scale bar represents 20 µm. Data representative of n = 8 App NL-F mice examined over 3 independent experiments. (b) Representative confocal image showing Spp1 mRNA in P2Y12 microglia associated with 6E10 + plaques in 15 mo App NL-F SLM. Scale bar represents 5 µm. Data representative of n = 3 App NL-F mice examined over 2 independent experiments. (c) Representative confocal images of SPP1 protein expression in CD206 + PVMs (arrow head) and CX3CR1 GFP myeloid cells of 3 mo Cx3cr1 GFP/WT SLM 86 . Scale bar represents 20 µm. Data are representative of n = 2 Cx3cr1 GFP/WT mice examined over at least 3 independent experiments. (d) Representative confocal image showing Spp1 mRNA expression occasionally colocalizing with pan-PVM markers Cd163 and Pf4 in 6 mo App NL-F