Fig. 5Figure 5
Appearance of leukocytes in CNS during PA-induced lung infection. a White blood counts in control and PA-infected animals (OD 600 0.3). Mann–Whitney test, P = 0.016 vs. control (uninfected) group. b Neutrophil and monocyte counts in blood obtained from control and PA-infected mice (OD 600 0.3) measured by ProCyte One hematology analyzer. Mann–Whitney test, P = 0.04, P = 0.03 vs. control (uninfected) group. c Flow cytometry analysis of brain tissue homogenates labeled with anti-CD45 and anti-CD11b antibodies with gating on CD45 + cells. Quantitative analyses of ( d ) infiltrated leukocytes and ( e ) microglia in control and PA infected mice. Mann–Whitney test, P = 0.03 vs. control (uninfected) group. In all graphs, each point indicates data from an individual animal, bar graphs (columns and error bars) show mean ± S.E.M. f Representative confocal images of infiltrated leukocytes (CD45 + ; green) in the brain from a control (left) and a PA-lung infected mouse (right). DAPI (nu