Fig. 7Figure 7
The inhibition of NETs improved the top 1 modules associated proteins expression in the hippocampus. A Double labeling IF staining of VCL with ECs (CD31) after GSK484 treatment in the hippocampus (n = 6). B Quantification of the fluorescence intensity of VCL in CD31-positive area (n = 6). C Quantification of the mean fluorescence intensity of VCL in all area (n = 6). D Double labeling IF staining of Fermt1 with ECs (CD31) after GSK484 treatment in the hippocampus (n = 6). E Quantification of the fluorescence intensity of Fermt1 in CD31-positive area (n = 6). F Quantification of the mean fluorescence intensity of Fermt1 in all area (n = 6). NETs: Neutrophil extracellular traps; CLP: Cecal Ligation and Puncture; SAE: Sepsis-associated encephalopathy; IF: immunofluorescence. (* p < 0.05, *** p < 0.001, **** p < 0.0001; ### p < 0.001, #### p < 0.0001; *sham vs CLP, # CLP vs CLP + GSK484)