Fig. 9Figure 9
The inhibition of NETs formation alleviated the disruption of TJs and AJs by activating Wnt3/β-catenin/TCF4 signaling pathway. A Expression levels of Wnt/β-catenin/TCF4 signaling proteins in the hippocampus were detected by Western blot (n = 6). B – H The quantification analysis of Wnt/β-catenin/TCF4 signaling proteins expression levels (n = 6). I Wnt3 is predominantly secreted by neurons in the hippocampal CA1 region of septic mice. J Quantification of the mean fluorescence intensity of Wnt3 in CA1 region (n = 6). NETs: Neutrophil extracellular traps; CLP: Cecal Ligation and Puncture; SAE: Sepsis-associated encephalopathy; BBB: Blood–brain barrier; TJs: Tight junctions; AJs: adherens junction (ns: no significance, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001)