Fig. 7Figure 7
Functional validation of predicted phenotypic plasticity upon chemotherapy. A Analysis of PAX8 mRNA expression, influence and relative protein expression in U87MG, U87MG-R50, and U87MG-OFF. Top panel: Representative Western blot (WB) detection of PAX8 protein and its predicted activity based on the regulatory network (colored dots under the WB), along with quantitative analysis ( n > 3) of its relative protein level. Unprocessed scans of the WB are provided in Supplementary Fig. 3 . Bottom panel: mRNA expression and influence barplots of PAX8 and detection of the PAX8 protein by immunofluorescence in the same samples analyzed by WB. B mRNA expression levels of NKX2.5 in primary and recurrent gliomas, as analyzed using the Chinese Glioma Genome Atlas (CGGA, http://www.cgga.org.cn/index.jsp ) cohort. NKX2.5 expression is significantly higher in grade IV gliomas compared to grade III and grade II gliomas. C Survival analysis of recurrent GBM patients (grade IV) based on NKX2.5 exp