Fig. 1Figure 1
Up-regulation of ZFP64 in DOX-resistant TNBC tissues was positively associated with high histone lactylation level. ( A & B ) Pan-Kla level in DOX-resistant/sensitive TNBC tissues was evaluated by immunofluorescence staining. ( C ) The protein levels of pan-Kla and H3K18la in DOX-resistant/sensitive TNBC tissues were assessed by Western blotting. ( D ) RT-qPCR analysis of ZFP64 mRNA expression in DOX-resistant/sensitive TNBC tissues. ( E ) Pearson correlation test analyzed the correlation between ZFP64 and lactylation levels. ( F ) Western blotting determined ZFP64 protein abundance in DOX-resistant/sensitive TNBC tissues. ( G ) Co-localization of ZFP64 and H3K18la in DOX-resistant/sensitive TNBC tissues was observed by immunofluorescence staining. Student’s t test was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001