Fig. 2Figure 2
ZFP64 expression was increased by CAFs-mediated histone lactylation. ( A & B ) ZFP64 expression was measured by RT-qPCR and Western blotting in TNBC cells co-cultured with or without CAF/S or CAF/R were treated with or without 20 mM oxamate or 25 mM LA for 48 h. ( C ) Western blotting analysis of Pan-Kla and H3K18la protein levels in TNBC cells co-cultured with or without CAF/S or CAF/R were treated with or without 20 mM oxamate or 25 mM LA for 48 h. ( D ) ChIP assay validated the interaction between H3K18la and ZFP64 promoter in TNBC cells co-cultured with or without CAF/S or CAF/R were treated with or without 20 mM oxamate or 25 mM LA for 48 h. n = 3. One-way ANOVA was performed for statistical analysis. * p < 0.05, ** p < 0.01, *** p < 0.001