Fig. 3Figure 3
ZFP64 knockdown counteracted CAFs-induced DOX insensitivity in TNBC cells. ( A ) CCK-8 determined the viability of DOX-treated TNBC cells co-cultured with or without CAF/R in combination with or without transfection with shNC or shZFP64. ( B ) Cell death was evaluated by PI staining via flow cytometry in DOX-treated TNBC cells co-cultured with or without CAF/R in combination with or without transfection with shNC or shZFP64. ( C - E ) MDA, Fe 2+ , and GSH levels were detected in DOX-treated TNBC cells co-cultured with or without CAF/R in combination with or without transfection with shNC or shZFP64. ( F ) Lipid ROS production was detected by C11-BODIPY 581/591 probe via flow cytometry in DOX-treated TNBC cells co-cultured with or without CAF/R in combination with or without transfection with shNC or shZFP64. ( G ) Mitochondrial morphological changes were determined by TEM in DOX-treated TNBC cells co-cultured with or without CAF/R in combination with or without transfection with shNC o