Fig. 4Figure 4
ZFP64 directly bound to the promoters of GCH1 and FTH1 to promote their transcription. ( A ) Expression levels of ferroptosis-related molecules in TNBC cells were detected by RT-qPCR after transfection with shNC or shZFP64 for 48 h. ( B ) FTH1 level in DOX-resistant/sensitive TNBC tissues was evaluated by RT-qPCR. ( C ) Pearson correlation test detected the correlation between ZFP64 and FTH1 levels in DOX-resistant TNBC tissues. ( D ) RT-qPCR analysis of GCH1 level in DOX-resistant/sensitive TNBC tissues. ( E ) Pearson correlation test evaluated the correlation between ZFP64 and GCH1 levels in DOX-resistant TNBC tissues. ( F & G ) GCH1 and FTH1 expression was measured by RT-qPCR and Western blotting in DOX-treated TNBC cells co-cultured with or without CAF/R together with or without transfection with shNC or shZFP64. ( H - J ) JASPAR database predicted the potential binding sites of ZFP64 to GCH1 or FTH1 promoter. ( K ) The direct binding of ZFP64 to GCH1 or FTH1 promoter in TNBC cells