Fig. 5Figure 5
FTH1 or GCH1 overexpression abolished shZFP64-mediated promotion in ferroptosis and DOX sensitivity in TNBC cells. ( A - D ) TNBC cells were transfected with vector, FTH1 or GCH1 overexpressing plasmid for 48 h. FTH1 and GCH1 expression in TNBC cells was assessed by RT-qPCR and Western blotting. ( E ) CCK-8 assay detected the viability of TNBC cells transfected with shZFP64 together with FTH1 or GCH1 overexpressing plasmid in the presence of DOX. ( F ) Cell death was determined by PI staining via flow cytometry in TNBC cells transfected with shNC or shZFP64 together with FTH1 or GCH1 overexpressing plasmid in the presence of DOX. ( G - I ) MDA, Fe 2+ , and GSH levels were measured in TNBC cells transfected with shNC or shZFP64 together with FTH1 or GCH1 overexpressing plasmid in the presence of DOX. ( J ) Lipid ROS production was assessed by C11-BODIPY 581/591 probe via flow cytometry in TNBC cells transfected with shNC or shZFP64 together with FTH1 or GCH1 overexpressing plasmid in th