Fig. 1Figure 1
GAS6 KO mice were more susceptible to JEV infection peripherally. Gas6 −/− and WT mice were infected with JEV at 10 6 PFU via foot pad injection. A, B, C. The survival rate ( A ), behavioural scores ( B ), and weight ( C ) of each group were recorded daily (gas6 KO n = 11; WT n = 8). D, F. The viral loads in gas6 −/− and WT mouse brains at 3 dpi ( D ) and 5 dpi ( F ) were detected using qPCR (gas6 −/− n = 5; WT n = 6). Data were normalised to β-actin and the relative change of viral copies was calculated based on the viral level of WT- d3- 1 or WT- d5- 1. Data are shown as mean ± SD. E, G. Relative expression of proinflammatory cytokines (TNF-α, CCL2, IFN-γ, IL-1β, IL-6) in gas6 −/− and WT mouse brains at 3 dpi ( E ) and 5 dpi ( G ) were detected by qPCR (gas6 −/− n = 5; WT n = 6). The data were normalised to β-actin and the relative change of expression was calculated based on the level of WT-d3-1 or WT-d5-1. Data are shown as mean ± SD