Fig. 1Figure 1
Characteristic expression of MTH1 in human gastric cancer tissues and ten digestive tract cancer cell lines. a The total RNA from fresh human gastric cancer tissues was isolated by RNApure Tissue&Cell Kit. The mRNA level of MTH1 in human gastric cancer (Increased, n = 21, Decreased, n = 6 and Unchanged, n = 8) and adjacent normal tissues (Con, n = 35) was determined by RT-PCR. The protein levels of MTH1 in human gastric (GC) and adjacent normal (GN) tissue sections were determined by immuohistochemistry (IHC) staining. The integral optical densities (IOD) were analyzed by Image-Pro Plus 6.0 software. b Representative IHC pictures of GC and GN. Scale bars, 50 μm. c IODs of GC and GN. n = 10 for each group. The cells from esophageal cancer cell lines: KYSE-450, EC109 and EC9706 ( d , e ), liver cancer cell lines: SMMC-7721, HepG2 and ZIP177 ( f , g ), gastric cancer cell lines: MGC-803, HGC-27, SGC-7901 and MKN45 ( h , i ), as well as the corresponding normal cell lines