Fig. 5Figure 6
RBG Inhibits Inflammation and NLRP3 Inflammasome Assembly Induced by LPS + ATP in BMDMs. (A and B) Concentrations of IL18 and IL1β in BMDM culture supernatants (n = 8). (C–H) Relative mRNA expression levels of NLRP3, NEK7, ASC, Caspase-1, IL18, and IL1β were measured in BMDMs (n = 4). (I-Q) Western blot analysis and statistical analysis of NLRP3, ASC total protein, ASC oligomerization, NEK7, Caspase-1, Cleaved Caspase-1, Pro IL18, and IL18 (n = 3). (R, U, V, W, X) Representative immunofluorescence images and statistical analysis of NLRP3, ASC, and Caspase-1 in BMDM (n = 3). (S and T) Representative images of NF-κB immunofluorescence in BMDMs. Ratio of NF-κB relative fluorescence intensity between the nucleus and cytoplasm (n = 3). * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001; ns indicates not significant.