Figure 1Figure 1
Neutrophils infiltrating and extruding extracellular traps (NETosis) in NMO mouse spinal cord. ( A ) Experimental design: catheter inserted via cisterna magna into L4 subarachnoid space (yellow circle, Evans blue verification). Subcutaneous osmotic pump continuously infuses AQP4-IgG or control mouse IgG (1.2 μg/d, in 12 μL). Rotarod motor training, days –3, –2, –1; testing, days 0, +1, +2, +3, +4, and +5. Terminal transcardiac perfusion: cord harvested for immunohistochemical and flow cytometric analyses. ( B ) Cord-infiltrating neutrophils (Cytek analysis), IgG infusion days 1, 3, and 5. Control mice (first panel) infused 3 days with normal mouse IgG. AQP4-IgG recipients: panels 2–4 (3 mice/group). t-SNE, t-distributed stochastic neighbor embedding. ( C – E ) IgG infusion day 3: representative images ( C and D ) and quantification ( E ) of neutrophils (Ly6G + MPO + ) in lumbar parenchyma (4 mice/group). Higher magnification box in C shows neutrophils (magenta) and nuclei (blue). (